Nah, Two-Face is dead… Which I think is a shame as he is one of my favourites from Batman Rogue Gallery… Riddler for sure (rumoured Johnny Deep), with a possibly recast Joker (which if they did, I think they should make a slight reinvention of the Joker, like a Madonna reinvention... as in he has a slightly new look and Demeanour. As though he'd become a new person, also to respect Ledger's Joker, by not having some copycat actor in the role) and another Scarecrow cameo would be cool. Penguin would definitely be in the mix as a Gangster type…
I’d love to see a well done and tragic Mr Freeze characterisation, but he wouldn’t fit in the grounded reality of the new Franchise. But I’d love to see that. I think if they made link to Superman’s or another super-powered hero’s universe in the next movie, so the viewer would understand that Batman is apart of a Meta-Human world, they might be able to suspend disbelieve for Mr Freeze in the same way they accepted Two-face’s impossible scaring… Which, if it happened in reality would kill him quite quickly; because of disease and bacteria around. Unless he was in a germ-free bubble until he healed in Hospital and developed a new outer skin!
Also, if they brought in the more expanded levels of dis-belief, they could bring in the idea of the Lazarus Pit for Ra's Return! As though he was killed in the first movie and his body was taken to a Pit by one of his subordinates...
Just a couple of ideas!
But in all Honesty, because of the upped Ante of the Police chasing Batman at the end of the Dark Knight (GOD! i Can't wait for the Blu-ray on Wednesday in Australia!!!) I can only think there will be a lot of cat and mouse between the Batman chasing the villian, whilst being chased by the Police at the same time! Which will make for some cool action I'd think! Like the Construction Chase scene in Batman: Mask of the Phantasm cartoon feature!!!